Dear Salman,
I have a quick question for you, and I hope you take it as intended. Given the attacks on America and Israel, it is only reasonable to expect us to question the intentions of those who belong to any group that cries for our destruction.
Can you tell me about this site? Is this standard? How many within the Muslim community support stuff like this:
http://www.worldtribune.com/worldtribune/06/front2454022.0881944443.html
I'm really not trying to be snotty, but these things are legitimate concerns. Thank you for any insight you can give me.
--
SO SAYS WARCHICK, Resa LaRu Kirkland
Columnist/Writer/Speaker/Military Historian/The Anti-Feminist!
Agree with RESA at: http://www.warchick.com
Hippies, commies, and femmies opinions altered here!!
STAND WITH ISRAEL! FREE NORTH KOREA!!
POW/MIA: Bring them home or send us back!
Hi Resa,
You are right in that we should question and be on the look out for any group
that cries for our destruction. There are religious, geopolitical, social and
economic issues that are all partly to blame for the hatred and destruction
that has been spawned at the start of this century. Lack of education and
homogeneity of populations also play a role (i.e. some have never lived
amongst
anyone but their own kind, who practice the same religion). As you know, my
family and I have been very focused on assimilating well into American society
and educating ourselves. Clearly, the American lives that are being lost in
Iraq and the hatred towards us by these so-called 'Muslims' is very
disturbing,
not just for you, but also for me and my family. I don't want to be subjected
to random suicide attacks, because you and I both know that when these people
kill, they kill without looking to see who is getting hurt. Of course, the
world trade center had people from all over the world.
Your question is very reasonable indeed. I can tell you that as in every
religion, there are liberals, conservatives, people in between and then
ridiculous fanatics. My family and I are liberals in a sense that we believe
Islam, Christianity and Judaism are God's religions and they are to be honored
and respected. Of course, we believe in general tolerance for all
religions. I associate with people of all faiths and in my experience
in America, I have
never met people who want to hurt others. I have met people who have
fundamental ideological differences, but many people can have that without
wanting to kill others.
Resa, clearly there are many people in the world who want to hurt us
Americans. And, it breaks my heart that many of these people are considered
'Muslim'. From my readings and experience, Islam in its core tenets is not
violent and very reasonable. These fanatics have interpreted many
things wrong
and though I don't have enough knowledge to give you a detailed historial and
political explanation, I can say that I have practiced a liberal version of
Islam well while associating with great rapport with Christians, Hindus, Jews,
Muslims and people of other faiths.
I don't know whether many Muslims in US support destruction of others, but in
my experience of 15 years in America, it seems to be a small percentage. I
have no idea what that violent percentage is except that these people have
infiltrated our way of living and peace and should be thoroughly investigated
and expelled. Maybe the percentage is higher when it comes to not supporting
American involvement in Iraq and Afghanistan, but I believe that a high
percentage of America in general doesn't support the war and the casualties
that we are experiencing. But, Pakistanis (my country of origin) for instance
are more supportive of the war in Iraq (and hence Allies of America)
since many
from Pakistan don't want any terrorism on their Afghani border and America is
helping them eradicate the violent component.
The site you emailed me about is by no means standard I think. What is
written on it is outrageous! Don't these people want to contribute something
to the world besides death? What is violence going to solve? What is killing
people going to solve? Violence and fighting have existed throughout history,
but where has it gotten us? I advocate for tolerance and great dialogue and I
believe that the Muslims I know advocate the same.
I only want one thing: To be safe, happy and living. If God forbid, in the
future, America is constantly attacked by terrorists, no matter what religion
they claim they are from, we must all join in speaking and acting against the
evil. Peace, goodness and life should matter more to us than anything else.
My answer is probably not very useful to you since I don't know of anyone who
even considers hurting others, but I wanted to give you the opinion of a
liberal Muslim, one who really wants to live peacefully and productively with
people of all faiths and backgrounds.
Salman
WARCHICK is a military historian/conservative commentator. She is a frequent guest on multiple talk-radio shows and is author of the forthcoming "Forgotten Warrior" series. She coined the phrases "Political Castration"--nothing correct about it--and ISLAMMUNISM--the secret combination of Islam/Communism. She sounds the war cry on North Korea and its unholy alliance with terrorism. She has a degree in Education but stands firm against the NEA. WARCHICK has been an online favorite since 2002.
Monday, February 19, 2007
Wednesday, February 14, 2007
***UPDATE***Letters from Salman and Ramzan Khetani
Salman is Faisal's brother and Ramzan is his father. Please read and respond.
Hello Resa,
My name is Salman Khetani and I am Faisal Khetani's brother. I was told by my father that you had inquired about the nature of my research, its scope and implications for the society at large. I have decided to respond to you because I want you and your readers to know about the types of opportunities I have been given in the US and the steps I have taken to reciprocate. My family and I immigrated to the US over a decade and a half ago in search of better professional and academic opportunities. We wanted to better ourselves through education and become productive members of society. I personally wanted to get the best education I could get and realize my vision of becoming a leader in science and engineering. Even in adolescence, I realized that I had the innate talent and drive to contribute to this world and improve the lives of people through my professional endeavors.
When I first started high school in the US, I had some difficulties with the transition, especially in terms of a slight language barrier (I knew how to read and write English, but didn't speak it very often before coming to the US). My parents, peers and wonderful teachers coached me through my transition period. They encouraged me and assured me that with hard work and struggle, I could overcome some of my initial problems. I was never discriminated against for my accent or darker color. In fact, I was well respected by most people I came into contact with at my high school and in the general community. I will always remember my mentors (teachers, soccer and tennis coaches, peers) in high school as they were my first impression of the graciousness of the American people. I assimilated well into the American society, made many friends, had an active social network, did decent amount of volunteer work to give back to the community and after 3 years, graduated as Valedictorian of my high school with a 4.0/4.0 GPA.
I received a scholarship to go to college where I pursued two Bachelor of Science degrees in Electrical Engineering and Biomedical Engineering. Once again, I had a wonderful experience. Not only did I learn the state-of-the-art in Engineering and Science, I used my skills to contribute to neighborhood organizations that served educational and other important needs of underprivileged youth and adults. In college, I had amazing professors as mentors, who instilled in me the value that with hard work, perseverance and a vision for the future, I could become a highly skilled and productive member of society, one who tries to improve the health and lives of people. They saw core talent in me from early on, talent that even I did not know existed. I also did substantial internships in industrial settings while I was in College and there too, I met the most brilliant, but incredibly generous people. Thanks to the financial generosity of my parents, mentorship of professors in school and coworkers in industry, support of peers, and my own hard work, I graduated college with two degrees and a 4.0/4.0 GPA.
Due to my high academic performance in college, I was awarded a National Science Foundation graduate fellowship to pursue doctorate work in Biomedical Engineering, which is also referred to as Bioengineering. I successfully defended and received my PhD in 2006. My research has focused on developing sophisticated laboratory models of liver tissues using liver cells from mostly animals and in some instances from brain-dead individuals (who have donated their organs for research). The overarching goal of my work is to use these body-like laboratory models for a variety of applications, including a) cell-based therapies for liver disease (i.e. bio-artificial liver devices that can keep a patient alive while they await a whole organ transplant), b) to investigate liver physiology and elucidate underlying mechanisms, and c) use in discovery and development of pharmaceuticals for detecting potentially problematic and toxic compounds before they reach patients. As you may know, many drugs reach the market and get withdrawn due to unforeseen toxicity to the liver or other organs in otherwise perfectly healthy individuals. Thus, the goal of my research is to make sure that such tragedies don't happen and that laboratory models using live cells from either animals or organ donors can predict problems much earlier in the drug development cycle. Therefore, as you can see, my research is aimed at helping society and not harming it. My work has been published in both scholarly journals and in lay press. I have included links and abstracts of these articles for your review below. All of these are available at www.pubmed.org which is a national archiving service. I hope that someday my work will be adopted by the pharmaceutical industry to make cheaper and above all, safer drugs for patients. Though I didn't comment explicitly on the amazing mentorship I received during graduate school, I wanted to just comment that getting a PhD is intimately tied to great mentorship and a great cohort of colleagues. I too had the great fortune to benefit from these aspects. In fact, nothing I accomplished in graduate school was in isolation. It was due to extensive technical help and scholarly advice from my supervisor and my colleagues. In spite of being involved in research over the last 6 years, I have only begun to realize my vision for the future. I plan to continue to use my engineering and biology skills sets to do cutting edge research and make important discoveries in a variety of different arenas in both academic and industrial settings.
I hope the narrative above provides you with some indication of the type of life I have lived so far and what my vision for the future is. I would like to reiterate to you that I want to help America and the World at large with my skills and research. I sincerely hope that I will continue to get opportunities which will allow me to accomplish my goals. For over a decade and half, I have had great experiences both academically and in the larger communities of the several states I have lived in. I love America, the diversity of cultures and religions here, and the generosity of the people. I learn from this diversity each and every day and I believe that I do my best to contribute with what I have. I understand that you wanted to probe deeper into what I do and if I was engaged in any so-called 'double work'. I would like to assure you that my family and I are humble, peace-loving and productive members of society. Regardless of our religion, we want nothing but the best for the US, of which we have been citizens of for almost a decade now.
Sincerely,
Salman R Khetani
----------------------------------------------------------------------
http://www.technologyreview.com/Biotech/16462/
-------------------------------------------------------------------------
In Vitro Liver Tissue Model Established from Transgenic Mice: Role of HIF1alpha on Hypoxic Gene Expression.
Allen JW, Khetani SR, Johnson RS, Bhatia SN.
Department of Bioengineering, University of California at San Diego, La Jolla, California., Current affiliation: David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California.
The instability of the hepatocyte phenotype in vitro has limited the ability to quantitatively investigate regulation of stress responses of the liver. Here, we adopt a tissue-engineering approach to form stable liver tissue in vitro by forming collagen "sandwich" cultures of transgenic murine hepatocytes harboring a regulatory gene of interest flanked by loxP sites. The floxed gene is excised in a subset of cultures by transfection with adenovirus carrying the gene for Cre-recombinase, thereby generating wild-type and null liver tissues from a single animal. In this study, we specifically investigated the role of hypoxia inducible factor 1 alpha (HIF-1alpha) in the hepatocellular response to hypoxia. Using high-density oligonucleotide arrays, we examined genome-wide gene expression after 8 h of hypoxia in wild-type and HIF- 1alpha null hepatocyte cultures. We identified more than 130 genes differentially expressed under hypoxia involved in metabolic adaptation, angiogenic signaling, immediate early response, and cell cycle regulation. Real-time polymerase chain reaction analysis verified that known hypoxia-responsive genes such as glucose transporter-1 and vascular endothelial growth factor were induced in a HIF-1alpha-dependent manner under hypoxia. Our results demonstrate the potential to integrate in vitro tissue models with transgenic and microarray technologies for the study of physiologic stress responses.
PMID: 17020444 [PubMed - as supplied by publisher]
--------------------------------------------------------------------------------
Curr Opin Biotechnol. 2006 Oct;17(5):524-31. Epub 2006 Sep 15. Related Articles, Links
Engineering tissues for in vitro applications.
Khetani SR, Bhatia SN.
Harvard MIT Division of Health Sciences and Technology/Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E19-502D, Cambridge, MA 02139, USA.
Engineered tissues can be employed for studies on the fundamental mechanisms of embryology and adult physiology and for investigating the evolution of disease processes. They also provide platforms to evaluate the behavior of new chemical entities in drug development. The recent development of three specific technologies has greatly facilitated the engineering of tissues for in vitro applications: the microfabrication tools that serve to both define the cellular microenvironment and enable parallelization of cell-based assays; synthetic, tunable hydrogels to create three-dimensional microenvironments; and bioreactors to control nutrient transport and fluid shear stress. Furthermore, convergence of these tools is providing investigators with the opportunity to construct and study tissues in vitro with unprecedented levels of sophistication.
Publication Types:
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
PMID: 16978857 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------------
Toxicol Sci. 2005 Mar;84(1):110-9. Epub 2004 Dec 8. Related Articles, Links
In vitro zonation and toxicity in a hepatocyte bioreactor.
Allen JW, Khetani SR, Bhatia SN.
Department of Bioengineering and Medicine, University of California at San Diego, La Jolla, California 92093-0412, USA.
The complex architecture of the liver is intertwined with its response to xenobiotic compounds. In particular, hepatocyte subpopulations are distributed along the sinusoid in zones 1 to 3, leading to prototypical "periportal" and "centrilobular" patterns of cell death in response to a toxic insult. In vitro models that more closely represent these zones of sub-specialization may therefore be valuable for the investigation of hepatic physiology and pathophysiology. We have established a perfused hepatocyte bioreactor that imposes physiologic oxygen gradients on co-cultures of rat hepatocytes and non-parenchymal cells, thereby producing an in vitro model of zonation. In order to predict and control oxygen gradients, oxygen transport in a parallel-plate bioreactor containing co-cultures was first mathematically modeled and experimentally validated. Co-cultures exposed to these physiologic oxygen gradients demonstrated regionally heterogeneity of CYP2B and CYP3A protein that mimics the distribution seen in the zonated liver. The distribution of CYP expression in the bioreactor was shown to vary with exposure to different chemical inducers and growth factors, providing a potential platform to study physiologic zonal responses. In order to explore zonal hepatotoxicity, bioreactors were perfused with APAP (acetominophen) for 24 h, resulting in maximal cell death at the low-oxygen outlet region similar to centrilobular necrotic patterns observed in vivo. This hepatocyte bioreactor system enables further in vitro investigation into zonation-dependent phenomena involving drug metabolism and toxicity.
Publication Types:
In Vitro
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
PMID: 15590888 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------------
Hepatology. 2004 Sep;40(3):545-54. Related Articles, Links
Exploring interactions between rat hepatocytes and nonparenchymal cells using gene expression profiling.
Khetani SR, Szulgit G, Del Rio JA, Barlow C, Bhatia SN.
Department of Bioengineering at the University of California-San Diego, La Jolla, CA 92093-0412, USA.
Cocultivation of primary hepatocytes with a plethora of nonparenchymal cells (from within and outside the liver) has been shown to support hepatic functions in vitro. Despite significant investigation into this phenomenon, the molecular mechanism underlying epithelial-nonparenchymal interactions in hepatocyte cocultures remains poorly understood. In this study, we present a functional genomic approach utilizing gene expression profiling to isolate molecular mediators potentially involved in induction of liver-specific functions by nonparenchymal cells. Specifically, primary rat hepatocytes were cocultivated with closely related murine fibroblast cell types (3T3-J2, NIH-3T3, mouse embryonic fibroblasts) to allow their classification as "high," "medium," or "low" inducers of hepatic functions. These functional responses were correlated with fibroblast gene expression profiles obtained using Affymetrix GeneChips. Microarray data analysis provided us with 17 functionally characterized candidate genes in the cell communication category (cell surface, extracellular matrix, secreted factors) that may be involved in induction of hepatic functions. Further analysis using various databases (i.e., PubMed, GenBank) facilitated prioritization of the candidates for functional characterization. We experimentally validated the potential role of two candidates in our coculture model. The cell surface protein, neural cadherin (N-cadherin), was localized to hepatocyte-fibroblast junctions, while adsorbed decorin up-regulated hepatic functions in pure cultures as well as cocultures with low-inducing fibroblasts. In the future, identifying mediators of hepatocyte differentiation may have implications for both fundamental hepatology and cell-based therapies (e.g., bioartificial liver devices). In conclusion, the functional genomic approach presented in this study may be utilized to investigate mechanisms of cell-cell interaction in a variety of tissues and disease states. Copyright 2004 American Association for the Study of Liver Diseases
Publication Types:
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
PMID: 15349892 [PubMed - indexed for MEDLINE]
And from Ramzan, Faisal and Salman's father:
Hi Resa - Greetings
I hope you got satisfactory answers to your queries regarding my son Salman’s research. Would you be kind enough to post email exchanges that took place between my son Salman’s and your good self so that fellow Americans can change their opinion about my family?
Thanks in anticipation. God bless you and our country United States.
Ramzan A. Khetani, P.E.
Project Manager/Electrical Engineer
Division of State Facilities
Wisconsin Department Of Administration
Tel: 608-267-4889
Fax: 608-267-2710
ramzan.khetani@wisconsin.gov
Web: www.doa.state.wi.us/dsf
Hello Resa,
My name is Salman Khetani and I am Faisal Khetani's brother. I was told by my father that you had inquired about the nature of my research, its scope and implications for the society at large. I have decided to respond to you because I want you and your readers to know about the types of opportunities I have been given in the US and the steps I have taken to reciprocate. My family and I immigrated to the US over a decade and a half ago in search of better professional and academic opportunities. We wanted to better ourselves through education and become productive members of society. I personally wanted to get the best education I could get and realize my vision of becoming a leader in science and engineering. Even in adolescence, I realized that I had the innate talent and drive to contribute to this world and improve the lives of people through my professional endeavors.
When I first started high school in the US, I had some difficulties with the transition, especially in terms of a slight language barrier (I knew how to read and write English, but didn't speak it very often before coming to the US). My parents, peers and wonderful teachers coached me through my transition period. They encouraged me and assured me that with hard work and struggle, I could overcome some of my initial problems. I was never discriminated against for my accent or darker color. In fact, I was well respected by most people I came into contact with at my high school and in the general community. I will always remember my mentors (teachers, soccer and tennis coaches, peers) in high school as they were my first impression of the graciousness of the American people. I assimilated well into the American society, made many friends, had an active social network, did decent amount of volunteer work to give back to the community and after 3 years, graduated as Valedictorian of my high school with a 4.0/4.0 GPA.
I received a scholarship to go to college where I pursued two Bachelor of Science degrees in Electrical Engineering and Biomedical Engineering. Once again, I had a wonderful experience. Not only did I learn the state-of-the-art in Engineering and Science, I used my skills to contribute to neighborhood organizations that served educational and other important needs of underprivileged youth and adults. In college, I had amazing professors as mentors, who instilled in me the value that with hard work, perseverance and a vision for the future, I could become a highly skilled and productive member of society, one who tries to improve the health and lives of people. They saw core talent in me from early on, talent that even I did not know existed. I also did substantial internships in industrial settings while I was in College and there too, I met the most brilliant, but incredibly generous people. Thanks to the financial generosity of my parents, mentorship of professors in school and coworkers in industry, support of peers, and my own hard work, I graduated college with two degrees and a 4.0/4.0 GPA.
Due to my high academic performance in college, I was awarded a National Science Foundation graduate fellowship to pursue doctorate work in Biomedical Engineering, which is also referred to as Bioengineering. I successfully defended and received my PhD in 2006. My research has focused on developing sophisticated laboratory models of liver tissues using liver cells from mostly animals and in some instances from brain-dead individuals (who have donated their organs for research). The overarching goal of my work is to use these body-like laboratory models for a variety of applications, including a) cell-based therapies for liver disease (i.e. bio-artificial liver devices that can keep a patient alive while they await a whole organ transplant), b) to investigate liver physiology and elucidate underlying mechanisms, and c) use in discovery and development of pharmaceuticals for detecting potentially problematic and toxic compounds before they reach patients. As you may know, many drugs reach the market and get withdrawn due to unforeseen toxicity to the liver or other organs in otherwise perfectly healthy individuals. Thus, the goal of my research is to make sure that such tragedies don't happen and that laboratory models using live cells from either animals or organ donors can predict problems much earlier in the drug development cycle. Therefore, as you can see, my research is aimed at helping society and not harming it. My work has been published in both scholarly journals and in lay press. I have included links and abstracts of these articles for your review below. All of these are available at www.pubmed.org which is a national archiving service. I hope that someday my work will be adopted by the pharmaceutical industry to make cheaper and above all, safer drugs for patients. Though I didn't comment explicitly on the amazing mentorship I received during graduate school, I wanted to just comment that getting a PhD is intimately tied to great mentorship and a great cohort of colleagues. I too had the great fortune to benefit from these aspects. In fact, nothing I accomplished in graduate school was in isolation. It was due to extensive technical help and scholarly advice from my supervisor and my colleagues. In spite of being involved in research over the last 6 years, I have only begun to realize my vision for the future. I plan to continue to use my engineering and biology skills sets to do cutting edge research and make important discoveries in a variety of different arenas in both academic and industrial settings.
I hope the narrative above provides you with some indication of the type of life I have lived so far and what my vision for the future is. I would like to reiterate to you that I want to help America and the World at large with my skills and research. I sincerely hope that I will continue to get opportunities which will allow me to accomplish my goals. For over a decade and half, I have had great experiences both academically and in the larger communities of the several states I have lived in. I love America, the diversity of cultures and religions here, and the generosity of the people. I learn from this diversity each and every day and I believe that I do my best to contribute with what I have. I understand that you wanted to probe deeper into what I do and if I was engaged in any so-called 'double work'. I would like to assure you that my family and I are humble, peace-loving and productive members of society. Regardless of our religion, we want nothing but the best for the US, of which we have been citizens of for almost a decade now.
Sincerely,
Salman R Khetani
----------------------------------------------------------------------
http://www.technologyreview.com/Biotech/16462/
-------------------------------------------------------------------------
In Vitro Liver Tissue Model Established from Transgenic Mice: Role of HIF1alpha on Hypoxic Gene Expression.
Allen JW, Khetani SR, Johnson RS, Bhatia SN.
Department of Bioengineering, University of California at San Diego, La Jolla, California., Current affiliation: David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California.
The instability of the hepatocyte phenotype in vitro has limited the ability to quantitatively investigate regulation of stress responses of the liver. Here, we adopt a tissue-engineering approach to form stable liver tissue in vitro by forming collagen "sandwich" cultures of transgenic murine hepatocytes harboring a regulatory gene of interest flanked by loxP sites. The floxed gene is excised in a subset of cultures by transfection with adenovirus carrying the gene for Cre-recombinase, thereby generating wild-type and null liver tissues from a single animal. In this study, we specifically investigated the role of hypoxia inducible factor 1 alpha (HIF-1alpha) in the hepatocellular response to hypoxia. Using high-density oligonucleotide arrays, we examined genome-wide gene expression after 8 h of hypoxia in wild-type and HIF- 1alpha null hepatocyte cultures. We identified more than 130 genes differentially expressed under hypoxia involved in metabolic adaptation, angiogenic signaling, immediate early response, and cell cycle regulation. Real-time polymerase chain reaction analysis verified that known hypoxia-responsive genes such as glucose transporter-1 and vascular endothelial growth factor were induced in a HIF-1alpha-dependent manner under hypoxia. Our results demonstrate the potential to integrate in vitro tissue models with transgenic and microarray technologies for the study of physiologic stress responses.
PMID: 17020444 [PubMed - as supplied by publisher]
--------------------------------------------------------------------------------
Curr Opin Biotechnol. 2006 Oct;17(5):524-31. Epub 2006 Sep 15. Related Articles, Links
Engineering tissues for in vitro applications.
Khetani SR, Bhatia SN.
Harvard MIT Division of Health Sciences and Technology/Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E19-502D, Cambridge, MA 02139, USA.
Engineered tissues can be employed for studies on the fundamental mechanisms of embryology and adult physiology and for investigating the evolution of disease processes. They also provide platforms to evaluate the behavior of new chemical entities in drug development. The recent development of three specific technologies has greatly facilitated the engineering of tissues for in vitro applications: the microfabrication tools that serve to both define the cellular microenvironment and enable parallelization of cell-based assays; synthetic, tunable hydrogels to create three-dimensional microenvironments; and bioreactors to control nutrient transport and fluid shear stress. Furthermore, convergence of these tools is providing investigators with the opportunity to construct and study tissues in vitro with unprecedented levels of sophistication.
Publication Types:
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
PMID: 16978857 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------------
Toxicol Sci. 2005 Mar;84(1):110-9. Epub 2004 Dec 8. Related Articles, Links
In vitro zonation and toxicity in a hepatocyte bioreactor.
Allen JW, Khetani SR, Bhatia SN.
Department of Bioengineering and Medicine, University of California at San Diego, La Jolla, California 92093-0412, USA.
The complex architecture of the liver is intertwined with its response to xenobiotic compounds. In particular, hepatocyte subpopulations are distributed along the sinusoid in zones 1 to 3, leading to prototypical "periportal" and "centrilobular" patterns of cell death in response to a toxic insult. In vitro models that more closely represent these zones of sub-specialization may therefore be valuable for the investigation of hepatic physiology and pathophysiology. We have established a perfused hepatocyte bioreactor that imposes physiologic oxygen gradients on co-cultures of rat hepatocytes and non-parenchymal cells, thereby producing an in vitro model of zonation. In order to predict and control oxygen gradients, oxygen transport in a parallel-plate bioreactor containing co-cultures was first mathematically modeled and experimentally validated. Co-cultures exposed to these physiologic oxygen gradients demonstrated regionally heterogeneity of CYP2B and CYP3A protein that mimics the distribution seen in the zonated liver. The distribution of CYP expression in the bioreactor was shown to vary with exposure to different chemical inducers and growth factors, providing a potential platform to study physiologic zonal responses. In order to explore zonal hepatotoxicity, bioreactors were perfused with APAP (acetominophen) for 24 h, resulting in maximal cell death at the low-oxygen outlet region similar to centrilobular necrotic patterns observed in vivo. This hepatocyte bioreactor system enables further in vitro investigation into zonation-dependent phenomena involving drug metabolism and toxicity.
Publication Types:
In Vitro
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
PMID: 15590888 [PubMed - indexed for MEDLINE]
--------------------------------------------------------------------------------
Hepatology. 2004 Sep;40(3):545-54. Related Articles, Links
Exploring interactions between rat hepatocytes and nonparenchymal cells using gene expression profiling.
Khetani SR, Szulgit G, Del Rio JA, Barlow C, Bhatia SN.
Department of Bioengineering at the University of California-San Diego, La Jolla, CA 92093-0412, USA.
Cocultivation of primary hepatocytes with a plethora of nonparenchymal cells (from within and outside the liver) has been shown to support hepatic functions in vitro. Despite significant investigation into this phenomenon, the molecular mechanism underlying epithelial-nonparenchymal interactions in hepatocyte cocultures remains poorly understood. In this study, we present a functional genomic approach utilizing gene expression profiling to isolate molecular mediators potentially involved in induction of liver-specific functions by nonparenchymal cells. Specifically, primary rat hepatocytes were cocultivated with closely related murine fibroblast cell types (3T3-J2, NIH-3T3, mouse embryonic fibroblasts) to allow their classification as "high," "medium," or "low" inducers of hepatic functions. These functional responses were correlated with fibroblast gene expression profiles obtained using Affymetrix GeneChips. Microarray data analysis provided us with 17 functionally characterized candidate genes in the cell communication category (cell surface, extracellular matrix, secreted factors) that may be involved in induction of hepatic functions. Further analysis using various databases (i.e., PubMed, GenBank) facilitated prioritization of the candidates for functional characterization. We experimentally validated the potential role of two candidates in our coculture model. The cell surface protein, neural cadherin (N-cadherin), was localized to hepatocyte-fibroblast junctions, while adsorbed decorin up-regulated hepatic functions in pure cultures as well as cocultures with low-inducing fibroblasts. In the future, identifying mediators of hepatocyte differentiation may have implications for both fundamental hepatology and cell-based therapies (e.g., bioartificial liver devices). In conclusion, the functional genomic approach presented in this study may be utilized to investigate mechanisms of cell-cell interaction in a variety of tissues and disease states. Copyright 2004 American Association for the Study of Liver Diseases
Publication Types:
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
PMID: 15349892 [PubMed - indexed for MEDLINE]
And from Ramzan, Faisal and Salman's father:
Hi Resa - Greetings
I hope you got satisfactory answers to your queries regarding my son Salman’s research. Would you be kind enough to post email exchanges that took place between my son Salman’s and your good self so that fellow Americans can change their opinion about my family?
Thanks in anticipation. God bless you and our country United States.
Ramzan A. Khetani, P.E.
Project Manager/Electrical Engineer
Division of State Facilities
Wisconsin Department Of Administration
Tel: 608-267-4889
Fax: 608-267-2710
ramzan.khetani@wisconsin.gov
Web: www.doa.state.wi.us/dsf
Monday, February 05, 2007
PREPARING FOR WAR IN THE STREETS...COULD THIS BE WHAT KHETANI'S GROUP IS UP TO?
Hat Tip: Michael (You know who you are!)
America's most gorgeous, not to mention credible, journalist Pamela Geller Oshry continues to expose the truths that our irresponsible, traitorous, liberal media refuses to acknowledge.
Preparing for War in the Streets of America
Can't reveal my sources on this but here's something to chew on.
"Public information officers or spokesman for law enforcement agencies are purposely downplaying and in some cases lying outright to the public about arrests of Muslims in the US," so said one Federal law enforcement official.
"We have been told what to say, and more important, what not to say, and warned not to deviate from the 'template.'
According to this source, this is causing some officers within various departments of federal agencies to become angry at the 'mandated political spin and pressure" created by 'orders from above." When asked who were giving such orders, this source could only say that it was from the "big boys in Washington DC."
Apparently this is killing morale and "everyone knows it's a joke." "It makes some of us sick, the politically correct shit we have to put up with."
The source whose father was an agent during the 1960s racial problems said what was happening then was nothing short of easy compared to now. "In those days at least there was some honesty in dealing with the problem, today there is none."
Specifically this source was responding to inquiries concerning one MOUSA M AMBUELAWI , 22, a Muslim fro St Charles Missouri who was charged buying or attempting to buy grenades, weapons and claymore mines to be used to "prepare for war."
MAN WAS ARMING FOR WAR St Louis Post Dispatch
A St. Charles man obtained fully automatic weapons and tried to buy as many explosives as possible in preparation for what an associate called "war," the FBI says in court documents.
He bought three rifles and a Claymore anti-personnel mine and negotiated for a case of hand grenades, documents obtained by the Post-Dispatch show.
Mousa M. Abuelawi, 22, of Franjoe Court, was arrested Dec. 29 and charged on complaints accusing him of three counts of illegal possession or distribution of a machine gun and conspiracy to violate machine gun statutes.
Abuelawi, a Palestinian immigrant free on $50,000 bond, could not be reached.
The context of the word "war" was not explained in court filings; the FBI declined to comment.
According to the agent, this case "fits a pattern we've been seeing."
"We're discovering more and more trafficking in arms and these kind of explosives, the stuff you see in the Middle East."
There are more and specific cases that illustrate that there are Muslim men here in the US that are "preparing to fight on the streets of our cities, or at least that's what they think they are going to do." And so they are "buying these weapons and even explosives, stealing them in many cases, preparing for this eventuality."
The men invloved in this movement commonly refered to as "the paintball jihad" Besides buying weapons and anything they can get their hands on, they are practicing urban combat.
"What is surprising to us, such as the trafficking in rocket launchers ( a reference to case in Albany) is that we are seeing more of this, more attempts to buy the kinds of weapons that used to be unheard of in law enforcement circles. It's a growing problem, one that we will see in streets before long, I'm afraid."
Posted by Pamela Geller Oshry on Monday, February 05, 2007 at 10:29 PM in
--
Support Paul Williams in his battle against terror and injustice......you may be next!
http://www.paulwilliamsdefensefund.com
'I have sworn on the altar of Almighty G-d eternal hostility toward every form of tyranny over the mind of man.' Thomas Jefferson
The things that will destroy America are prosperity-at-any-price, peace-at-any-price, safety-first instead of duty-first, the love of soft living, and the get-rich-quick theory of life." ----Theodore Roosevelt
America's most gorgeous, not to mention credible, journalist Pamela Geller Oshry continues to expose the truths that our irresponsible, traitorous, liberal media refuses to acknowledge.
Preparing for War in the Streets of America
Can't reveal my sources on this but here's something to chew on.
"Public information officers or spokesman for law enforcement agencies are purposely downplaying and in some cases lying outright to the public about arrests of Muslims in the US," so said one Federal law enforcement official.
"We have been told what to say, and more important, what not to say, and warned not to deviate from the 'template.'
According to this source, this is causing some officers within various departments of federal agencies to become angry at the 'mandated political spin and pressure" created by 'orders from above." When asked who were giving such orders, this source could only say that it was from the "big boys in Washington DC."
Apparently this is killing morale and "everyone knows it's a joke." "It makes some of us sick, the politically correct shit we have to put up with."
The source whose father was an agent during the 1960s racial problems said what was happening then was nothing short of easy compared to now. "In those days at least there was some honesty in dealing with the problem, today there is none."
Specifically this source was responding to inquiries concerning one MOUSA M AMBUELAWI , 22, a Muslim fro St Charles Missouri who was charged buying or attempting to buy grenades, weapons and claymore mines to be used to "prepare for war."
MAN WAS ARMING FOR WAR St Louis Post Dispatch
A St. Charles man obtained fully automatic weapons and tried to buy as many explosives as possible in preparation for what an associate called "war," the FBI says in court documents.
He bought three rifles and a Claymore anti-personnel mine and negotiated for a case of hand grenades, documents obtained by the Post-Dispatch show.
Mousa M. Abuelawi, 22, of Franjoe Court, was arrested Dec. 29 and charged on complaints accusing him of three counts of illegal possession or distribution of a machine gun and conspiracy to violate machine gun statutes.
Abuelawi, a Palestinian immigrant free on $50,000 bond, could not be reached.
The context of the word "war" was not explained in court filings; the FBI declined to comment.
According to the agent, this case "fits a pattern we've been seeing."
"We're discovering more and more trafficking in arms and these kind of explosives, the stuff you see in the Middle East."
There are more and specific cases that illustrate that there are Muslim men here in the US that are "preparing to fight on the streets of our cities, or at least that's what they think they are going to do." And so they are "buying these weapons and even explosives, stealing them in many cases, preparing for this eventuality."
The men invloved in this movement commonly refered to as "the paintball jihad" Besides buying weapons and anything they can get their hands on, they are practicing urban combat.
"What is surprising to us, such as the trafficking in rocket launchers ( a reference to case in Albany) is that we are seeing more of this, more attempts to buy the kinds of weapons that used to be unheard of in law enforcement circles. It's a growing problem, one that we will see in streets before long, I'm afraid."
Posted by Pamela Geller Oshry on Monday, February 05, 2007 at 10:29 PM in
--
Support Paul Williams in his battle against terror and injustice......you may be next!
http://www.paulwilliamsdefensefund.com
'I have sworn on the altar of Almighty G-d eternal hostility toward every form of tyranny over the mind of man.' Thomas Jefferson
The things that will destroy America are prosperity-at-any-price, peace-at-any-price, safety-first instead of duty-first, the love of soft living, and the get-rich-quick theory of life." ----Theodore Roosevelt
Sunday, February 04, 2007
Another update
Another update...and lie.
---------- Forwarded message ----------
From: Contact
Date: Jan 31, 2007 2:18 PM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
Resa,
I do not own that factory and it is not part of my lot. Please look up that factory's address and confirm with the City of West Allis... Please do call the City of West Allis rather than chasing this issue with speculation. Look up the factory's address, call them, and call the City...
As for my business, as you know I have multiple businesses and I need customer service reps who take phone calls, emails, generate invoices, do accounting, and deal with the manufacturer. The number of associates and members I have is for my business as a whole.
Google Earth is not giving you the true picture... Send someone to my house and take pictures of my property and and my backyard and you'll see that the factory is not at all a part of my lot. Instead of using Google Earth, why don't you people take the sensible route of looking up the address, calling up the City of West Allis, and figuring it out once and for all?
---------- Forwarded message ----------
From: Contact
Date: Jan 31, 2007 2:18 PM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
Resa,
I do not own that factory and it is not part of my lot. Please look up that factory's address and confirm with the City of West Allis... Please do call the City of West Allis rather than chasing this issue with speculation. Look up the factory's address, call them, and call the City...
As for my business, as you know I have multiple businesses and I need customer service reps who take phone calls, emails, generate invoices, do accounting, and deal with the manufacturer. The number of associates and members I have is for my business as a whole.
Google Earth is not giving you the true picture... Send someone to my house and take pictures of my property and and my backyard and you'll see that the factory is not at all a part of my lot. Instead of using Google Earth, why don't you people take the sensible route of looking up the address, calling up the City of West Allis, and figuring it out once and for all?
Still waiting
Here it is...this is the one I'm waiting to hear back from Mr. Khetani on, regarding the obvious metal building behind his house.
They grow bolder...
...as we fall asleep. Tennessee, what the hell....?
http://www.wmcstations.com/global/story.asp?s=5996486&ClientType=Printable
http://www.freerepublic.com/focus/f-news/1761584/replies?c=1553
Why don't we just GIVE them the country? That's what we're doing anyway, slowly and painfully...
http://www.wmcstations.com/global/story.asp?s=5996486&ClientType=Printable
http://www.freerepublic.com/focus/f-news/1761584/replies?c=1553
Why don't we just GIVE them the country? That's what we're doing anyway, slowly and painfully...
Picture this!
Date: Mon, 29 Jan 2007 07:20:47 -0800 (PST)
From: "Scott F. Cook"
Subject: picture This!
To: resalaru@warchick.com
I sent this to the fine folks at euphoricreality.com, and thought you might find it interesting too.
--
did some picture taking today. (as far as I know all attachments are scanned by yahoo and are virus-free). sorry the quality isn't better - i used my camera phone. These are all pretty non-descript buiildings, nothing really out of the ordinary. The last one is particularly interesting I think.
3028 N Maryland, duplex. The address of the property information I sent yesterday (I think). No parking on one side of the street, so I took this while driving past. Milwaukee east side, not far from UWM.
229 W Orchard, duplex? The property Faisal currently has for rent, and has listed on the Milwaukee craigslist. Milwaukee south side.
1020.jpg - 1020 E Pleasant. Apt building where Sajid Nasir lives. Milwaukee east side.
Sajid Nasir's mailbox, confirmation of residence.
2045 N Oakland. Apt building where Acccchmed Busra lives.
Another view, with moving truck. HMM.
The biggie. 9035 S. 49th St is one of these two houses. It is also the official listed address of Capital Renovations LLC, "d/b/a" of Bargain Suppliers. Per http://ccap.wicourts.gov, John Kenenakhone is listed to the address, who along with Ryan Pattee run "Commercial Bargains LLC". The connection? John K's phone number (and address) are listed on the Capital Renovations record, along with Faisal's sportsfitness@gmail.com address.
Ryan P is the only other employee of the Commercial Bargains, according to www.manta.com. http://ccap.wicourts.gov lists Ryan P.'s apt is 2nd St. downtown Milwaukee address. The business is on S. 4th St. in a warehouse on Milwaukee's south side. Why someone with a LLC at his home address be driving more than 13 mi & 15 min to another LLC?
I could not find at the time, 3270 W. American Dr., but later looking at http://local.live.com with the bird's eye feature I was on the right track - it was a condo complex.
Now the big question - what needs to be done with info?
From: "Scott F. Cook"
Subject: picture This!
To: resalaru@warchick.com
I sent this to the fine folks at euphoricreality.com, and thought you might find it interesting too.
--
did some picture taking today. (as far as I know all attachments are scanned by yahoo and are virus-free). sorry the quality isn't better - i used my camera phone. These are all pretty non-descript buiildings, nothing really out of the ordinary. The last one is particularly interesting I think.
3028 N Maryland, duplex. The address of the property information I sent yesterday (I think). No parking on one side of the street, so I took this while driving past. Milwaukee east side, not far from UWM.
229 W Orchard, duplex? The property Faisal currently has for rent, and has listed on the Milwaukee craigslist. Milwaukee south side.
1020.jpg - 1020 E Pleasant. Apt building where Sajid Nasir lives. Milwaukee east side.
Sajid Nasir's mailbox, confirmation of residence.
2045 N Oakland. Apt building where Acccchmed Busra lives.
Another view, with moving truck. HMM.
The biggie. 9035 S. 49th St is one of these two houses. It is also the official listed address of Capital Renovations LLC, "d/b/a" of Bargain Suppliers. Per http://ccap.wicourts.gov, John Kenenakhone is listed to the address, who along with Ryan Pattee run "Commercial Bargains LLC". The connection? John K's phone number (and address) are listed on the Capital Renovations record, along with Faisal's sportsfitness@gmail.com address.
Ryan P is the only other employee of the Commercial Bargains, according to www.manta.com. http://ccap.wicourts.gov lists Ryan P.'s apt is 2nd St. downtown Milwaukee address. The business is on S. 4th St. in a warehouse on Milwaukee's south side. Why someone with a LLC at his home address be driving more than 13 mi & 15 min to another LLC?
I could not find at the time, 3270 W. American Dr., but later looking at http://local.live.com with the bird's eye feature I was on the right track - it was a condo complex.
Now the big question - what needs to be done with info?
Emails with Faisal Khetani
From: Contact
Date: Jan 29, 2007 10:14 AM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
There is a factory behind my house, but it is not attached to my house and its in a completely different lot, which is completely unrelated to my residential property, and not at all a part of it. Please look up the address of that factory and look up their information to confirm that they are in no way related to my property and in no way affiliated with my company. If in doubt contact the City of West Allis to ask them what that factory is and what kind of products they produce.
Faisal
On 1/28/07, Resa Kirkland wrote:
Comment?
---------- Forwarded message ----------
From:
Date: Jan 28, 2007 2:54 PM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
BTW, Resa,
If you search his 106th St address in Google Earth, you will see that directly behind his house (maybe even attached to it) is one of those large half round metal buildings. If he is drop shipping, why does he need that?
P.O
Date: Jan 29, 2007 10:14 AM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
There is a factory behind my house, but it is not attached to my house and its in a completely different lot, which is completely unrelated to my residential property, and not at all a part of it. Please look up the address of that factory and look up their information to confirm that they are in no way related to my property and in no way affiliated with my company. If in doubt contact the City of West Allis to ask them what that factory is and what kind of products they produce.
Faisal
On 1/28/07, Resa Kirkland
Comment?
---------- Forwarded message ----------
From:
Date: Jan 28, 2007 2:54 PM
Subject: Re: From Faisal Khetani
To: Resa Kirkland
BTW, Resa,
If you search his 106th St address in Google Earth, you will see that directly behind his house (maybe even attached to it) is one of those large half round metal buildings. If he is drop shipping, why does he need that?
P.O
Google Earth placemarks
These are Google Earth "placemarks" where you can find locations using the Google Earth system. If you have Google Earth installed, you can double-click on the attached Placemark file and it will fly you to a location. If not, you will need to install Google Earth first (available at http://earth.google.com).
Bushra's Address
Khetani's Address
Nasir's Address
Address for Nasir & Bushra
Khetani's Capital Renovations Address
Another Khetani Address
Bushra's Address
Khetani's Address
Nasir's Address
Address for Nasir & Bushra
Khetani's Capital Renovations Address
Another Khetani Address
Thursday, February 01, 2007
WARCHICK REVOLUTION TONIGHT AT 7PM PACIFIC, WITH JIHAD WATCH'S ROBERT SPENCER!
Tonight's show is blessed to have on it the incomparable Robert Spencer of www.JihadWatch.com. You've seen Mr. Spencer on FOX News and Atlas Shrugs' Pamela Oshry!!! Now WARCHICK's got him, and you're invited.
Spread the word...tonight's show begins at 7PM Pacific time, 10PM Eastern Time, and call in number is (347) 996-5936.
To listen, go to www.blogtalkradio.com/WARCHICK and wait for the button to show up, then press PLAY.
Robert has been sounding the warning against Jihad for more than a decade now...this promises to be an informative and fascinating hour. DON'T MISS THIS INCREDIBLE OPPORTUNITY!
Keep the faith, bros, in all things courage, and no substitute for VICTORY.
--
SO SAYS WARCHICK, Resa LaRu KirklandColumnist/Writer/Speaker/Military Historian/The Anti-Feminist!
Agree with RESA at: http://www.warchick.com
Hippies, commies, and femmies opinions altered here!!
STAND WITH ISRAEL! FREE NORTH KOREA!!
POW/MIA: Bring them home or send us back!
Spread the word...tonight's show begins at 7PM Pacific time, 10PM Eastern Time, and call in number is (347) 996-5936.
To listen, go to www.blogtalkradio.com/WARCHICK and wait for the button to show up, then press PLAY.
Robert has been sounding the warning against Jihad for more than a decade now...this promises to be an informative and fascinating hour. DON'T MISS THIS INCREDIBLE OPPORTUNITY!
Keep the faith, bros, in all things courage, and no substitute for VICTORY.
--
SO SAYS WARCHICK, Resa LaRu KirklandColumnist/Writer/Speaker/Military Historian/The Anti-Feminist!
Agree with RESA at: http://www.warchick.com
Hippies, commies, and femmies opinions altered here!!
STAND WITH ISRAEL! FREE NORTH KOREA!!
POW/MIA: Bring them home or send us back!
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